Kuongororwa kweOxalic Acid Mumichero neMiriwo neHigh-Performance Liquid Chromatography (HPLC)
1. Nheyo yekuedza uye dhizaini yenzira
Semushonga weorganic acid unowanikwa mumichero nemiriwo, huwandu hweoxalic acid hunokanganisa zvakananga kuravira uye kukosha kwekudya kwechikafu. Kuedza uku kunoshandisa reversed-phase high-performance liquid chromatography (RP-HPLC). Pasi pemamiriro ezvinhu eacidic mobile phase, oxalic acid inoparadzaniswa nebaseline kubva kune zvinhu zvinokanganisa uchishandisa C18 chromatographic column. Chinoongorora ultraviolet chakaiswa pa210 nm chinoshandiswa pakuongorora huwandu, zvichibva pahunhu hweUV hwekunwa kwemapoka ecarboxyl mumamorekuru eoxalic acid.
2. Kugadzirira Kwakajairwa uye Muenzaniso
Nzira yekudiridza gradient inoshandiswa kugadzira mhinduro dzakajairika: kuyera nemazvo 25.0 mg ye oxalic acid dihydrate woideredza kusvika ku25 mL nemvura yakachena kwazvo kuti uwane mhinduro ye 1 mg/mL. Izvi zvinodzikiswa zvakatevedzana muzvikamu zvakatevedzana zvemhinduro dzakajairika pa 50, 100, 200, 400, uye 800 µg/mL, uye huwandu hwega hwega huchipinzwa muzvikamu zvitatu.
Pakugadzirira mienzaniso, kutorwa kwemushonga uchishandisa microwave kunoshandiswa: kuyera 5.00 g yemuchero/muriwo, kuwedzera 10 mL ye 0.1 mol/L hydrochloric acid solution, bvisa pa60°C ne microwave kwemaminitsi gumi, sefa nepa 0.45 µm membrane filter, uye unganidza filtrate kuti iongororwe.
3. Kugadzirisa Mamiriro EChromatographic
Chikamu chinofambiswa i 0.01 mol/L potassium dihydrogen phosphate buffer (pH 2.5)–acetonitrile (95:5), ine mwero wekuyerera we 0.8 mL/min uye tembiricha yekoramu ye 35°C. Kugadzirisa chiyero che acetonitrile kunoratidza kuti kana chikamu che organic chapfuura 10%, nguva yekuchengetedza ye oxalic acid inopfupika kusvika pasi pemaminitsi matatu, asi kukwirira kwemuswe kunoitika. Pasi pemamiriro ekupedzisira akagadziridzwa, nguva yekuchengetedza ye oxalic acid imaminitsi 4.2, zvichiita kuti pave nekuparadzaniswa kwakakwana kubva kune citric acid iri pedyo ne malic acid (resolution > 1.5).
4. Kusimbiswa kweNzira
Kuongororwa kwemhando yeLinear Range kunoratidza hukama hwakanaka pakati penzvimbo yepamusoro uye huwandu hwehuwandu huri pakati pe10–1000 µg/mL (R² = 0.9993). Muganho wekuona (LOD), unotsanangurwa nenzira yechiratidzo-kune-ruzha, i0.5 µg/mL. Muedzo dzekudzokorora, RSD yematanho matanhatu ekuongorora akafanana emusanganiswa wespinachi i1.8%. Kuedza kweSpike recovery pamatanho matatu (80%, 100%, uye 120%) kunoburitsa avhareji yekudzoka kwe98.2%, 102.4%, uye 97.8%, zvichiteerana, zvichisangana nezvinodiwa zvekuongorora huwandu.
5. Kuongororwa kweMienzaniso Chaiyo
Michero nemiriwo mitanhatu inowanikwa kutengeswa yakaedzwa: sipinachi (356 ± 12 mg/100 g), celery (215 ± 9 mg/100 g), domasi (18 ± 2 mg/100 g), apuro (6 ± 1 mg/100 g), bhanana (hazvina kuonekwa), uye broccoli (89 ± 5 mg/100 g). Kuenzanisa nenzira yenyika (GB 5009.277-2016) kunoratidza kuti kusiyana pakati penzira mbiri idzi kuri pasi pe5%, zvichiratidza kuvimbika kwenzira iyi. Kunyanya, nzira yeHPLC inoratidza kunzwisisika kwakanyanya kupfuura nzira dzechinyakare dzekuti titration pakuongorora samples dzisina kusimba.
6. Zvinyorwa Zvinokosha
Dzora pH zvakanyanya panguva yekurapwa kwemuenzaniso usati watanga. Kana pH yemushonga ichidarika 3, calcium oxalate precipitation ichaita kuti pave nemigumisiro yakaderera yebvunzo. Chikamu chinofamba chinofanira kugadzirwa chitsva zuva nezuva kudzivirira kunaya kwemunyu uye kuvharika kwemushonga. Mushure memajekiseni makumi maviri ega ega, sukurudza mushonga neacetonitrile-water (30:70) kwemaminitsi makumi matatu kudzivirira kuora kwekushanda kwemushonga. Kune sampuli dzine pigment (semuenzaniso, purple cabbage), zvinokurudzirwa kuwedzera danho rekuchenesa mushonga usati wabaya.
7. Mirayiridzo yekushandisa uye kuwedzera
Nzira iyi inogona kuwedzerwa kusvika pakuona oxalic acid muzviyero zvehupenyu zvakaita seweti neropa. Nekugadzirisa chimiro che mobile phase (semuenzaniso, kuwedzera tetrabutylammonium hydroxide), oxalic acid ne metabolites dzayo zvinogona kuonekwa panguva imwe chete. Pamwe chete ne mass spectrometry detector, nzira yakanyatsojeka yekuona trace inogona kuiswa. Muindasitiri yekugadzira chikafu, nzira iyi inogona kupa rutsigiro rwakakosha rwedata pakusarudzwa kwemhando dze low-oxalic acid uye kugadzirisa maitiro ekubika.
Zibo Anhao Chemical Co., Ltd. – Oxalic Acid
Zibo Anhao Chemical Co., Ltd. mutengesi akavimbika uye anotumira kunze kwenyika oxalic acid yemhando yepamusoro (yemhando yeindasitiri, 99.6% min) kubva kuShandong, China. Oxalic acid yedu icrystalline solid chena, inozivikanwawo se ethanedioic acid, ine molecular formula C₂H₂O₄. Ine kuchena kwakanyanya, tsvina shoma, uye mhando yakagadzikana, ichizadzisa zvinodiwa zvemachira, dehwe, simbi, rare earth processing, chemical cleaning, uye maindasitiri ekuvaka.
Tinopa oxalic acid isina mvura nedhihydrate, inowanikwa mumabhegi e25kg, mabhegi e50kg, uye mabhegi e1000kg. Nemaitiro akaomarara eQC, mishumo yakazara yekuyedza, uye zvirongwa zvekugovera zvinochinjika, tinovimbisa kuti kuendesa kwakasimba uye kushanda kwakasimba kune vatengi vepasi rose. Zvigadzirwa zvedu zvakatumirwa zvakanyanya kuSoutheast Asia, Europe, Africa, neAmerica, zvichiwana kuzivikanwa kwemhando yakavimbika uye mitengo inokwikwidzana.
Sarudza Zibo Anhao Chemical kuti uwane oxalic acid yakavimbika uye kuti uwane makemikari ehunyanzvi.
Sarudza Zibo Anhao Chemical ye oxalic acid yakachena kwazvo inoita kuti bhizinesi rako rishande zvakanaka uye rive rakakodzera. Taura nesu kuti uwane maodha akawanda kana kuti uwane mamwe mashoko ehunyanzvi: info@anhaochemical.com
